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KMID : 0903519690110010137
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1969 Volume.11 No. 1 p.137 ~ p.141
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Studies on the Isolation of Auxotrophic Mutants of Bacillus subtilis and Escherichia coli
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Lee Ke-Ho
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Abstract
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In order to obtain amino acids and nucleic acid derivatives from adenine auxotrophic mutants of Bacillus subtilis and Escherichia coli, vitamin, nucleic acid analogue, streptomycin as well as ultraviolet light were adopted for the production of adenine auxotrophic mutants and the results showed efficient production of desired mutants.
1. Ultraviolet ray(2530 Å 2080 erg/㎟) irradiation to Bacillus subtilis and E. cola at a distance of 30 ㎝ for 80-90 sec. and for 15-20 sec. respectively induced four and eight strains of auxotrophic mutants.
2. Treatment of aminopterine(200㎍/㎖) inhibited the growth of Bacillus sulitilis significantly but a subsequent irradiation of ultraviolet light at the above mentioned conditions induced six times as much mutants as compared to the irradiation alone. In case of E. coli a similar tendency was observed with treatment of streptomycin(200 ㎍/㎖) with doubled induction rate of adenine auxotropizic mutants as compared to the irradiation alone.
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